TLR5 Mouse Monoclonal Antibody [Clone ID: 85B152.5]

CAT#: TA336873

Mouse Monoclonal TLR5 Antibody (85B152.5)

Product Images

Flow Cytometry: TLR5 Antibody (85B152.5) TA336873 - Intracellular flow analysis of TLR5 in Ramos cells using 1 ug of TLR5 antibody. Shaded histogram represents Ramos cells without antibody; green represents isotype control antibody; red represents TLR5 antibody.

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  Flow Cytometry: TLR5 Antibody (85B152.5) TA336873 - Intracellular flow analysis of TLR5 in 10^6 human lymphocytes using 0.5 ug of NB200-571. The shaded histogram represents cells without antibody, green represents isotype control (BD), and red represents TLR5 antibody.

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  Flow Cytometry: TLR5 Antibody (85B152.5) TA336873 - Analysis using the PE conjugate of TA336873. Staining of TLR5 in 10^6 RAW cells using 0.5 ugs of PE-conjugated antibody. The black histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

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  Flow Cytometry: TLR5 Antibody (85B152.5) TA336873 - Analysis using the PE conjugate of TA336873. Staining of TLR5 in 10^6 human lymphocytes using 0.5 ugs of PE-conjugated antibody. The shaded histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

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  Flow Cytometry: TLR5 Antibody (85B152.5) TA336873 - Analysis using the FITC conjugate of TA336873. Staining of TLR5 in 10^6 cells using 0.5 ugs of FITC-conjugated antibody: A) Intracellular flow testing of human Ramos cells and B) cell surface flow testing of mouse RAW cells. The shaded (or black) histogram represents cells without antibody, green represents isotype control antibody, and red represents TLR5 antibody.

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Specifications

Product Data

• Clone Name: 85B152.5
• Applications: FC, WB
• Recommended Dilution: Flow (Cell Surface), Immunohistochemistry-Paraffin, Flow (Intracellular), Immunohistochemistry, Flow Cytometry: 1-3 ug/10^6 cells in 100 ul, Western Blot: 1-3 ug/ml
• Reactivities: Human, Mouse, Canine
• Host: Mouse
• Isotype: IgG2a
• Clonality: Monoclonal
• Immunogen: This antibody was developed against KLH-conjugated synthetic peptide corresponding to a portion of human TLR5. It will cross-react with mouse TLR5 (NP_003259).
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 100 kDa
• Gene Name: toll like receptor 5
• Database Link:
  NP_003259
  Entrez Gene 53791 MouseEntrez Gene 7100 Human
  UniProt ID O60602
• Background: The Toll-like receptor (TLR) family in mammal comprises a family of transmembrane proteins characterized by multiple copies of leucine rich repeats in the extracellular domain and IL-1 receptor motif in the cytoplasmic domain. Like its counterparts in Drosophila, TLRs signal through adaptor molecules and could constitute an important and unrecognized component of innate immunity in humans. The TLR family is a phylogenetically conserved mediator of innate immunity that is essential for microbial recognition. TLRs characterized so far activate the MyD88/interleukin-1 receptor-associated kinase (IRAK) signaling pathway. Thirteen homologs of TLRs (TLR1-13) have been described. Toll-like receptor 5 (TLR5) expression is upregulated following exposure to bacteria or to the TLR5 agonist, flagellin. Gram-negative bacteria, stimulate monocyte/macrophage cells in a TLR5-specific, CD14-independent manner. The TLR5 receptor thus appears to be the principal means by which the innate immune system recognizes flagellated bacterial pathogens.
• Synonyms: MELIOS; SLE1; SLEB1; TIL3
• Note: Reported use in Flow Cytometry (cell surface): see Wong et al, 2007 for details.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Pathogenic Escherichia coli infection, Toll-like receptor signaling pathway