Aurora A (AURKA) Mouse Monoclonal Antibody [Clone ID: 1F8]

CAT#: TA336917

Mouse Monoclonal Aurora A Antibody (1F8)

Product Images

ELISA: Aurora A Antibody (1F8) TA336917 - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

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  Immunocytochemistry/Immunofluorescence: Aurora A Antibody (1F8) TA336917 - Analysis of Hela cells using Aurora A mouse mAb (green). DRAQ5 fluorescent DNA dye (blue).

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  Western Blot: Aurora A Antibody (1F8) TA336917 - Analysis using Aurora A mouse mAb against HEK293 (1), Sw620 (2), MCF-7 (3), Jurkat (4), Hela (5), HepG2 (6), Cos7 (7) and PC-12 (8) cell lysates.

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  Flow Cytometry: Aurora A Antibody (1F8) TA336917 - Analysis of K562 cells using Aurora A mouse mAb (green) and negative control (purple).

Specifications

Product Data

• Clone Name: 1F8
• Applications: ELISA, FC, WB
• Recommended Dilution: Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Western Blot: 1:500-1:2000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000
• Reactivities: Human, Rat, Primate
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Aurora A expressed in E. coli. [UniProt# O14965]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 48 kDa
• Gene Name: aurora kinase A
• Database Link:
  NP_940835
  Entrez Gene 261730 RatEntrez Gene 6790 Human
  UniProt ID O14965
• Background: Aurora A plays a role in cell cycle regulation during anaphase and/or telophase, in relation to the function of the centrosome/spindle pole region during chromosome segregation. Aurora A plays a key role during tumor development and progression and is overexpressed in many human cancers including breast, ovarian and colorectal. Aurora A is viewed as a potential target for anticancer drug treatment.Tissue specificity: Highly expressed in testis and weakly in skeletal muscle, thymus and spleen. Also highly expressed in colon, ovarian, prostate, neuroblastoma, breast and cervical cancer cell lines.
• Synonyms: AIK; ARK1; AURA; BTAK; PPP1R47; STK6; STK7; STK15
• Note: This Aurora A (1F8) antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Oocyte meiosis