iNOS (NOS2) Mouse Monoclonal Antibody [Clone ID: 4E5]

CAT#: TA336918

Mouse Monoclonal iNOS Antibody (4E5)

Product Images

ELISA: iNOS Antibody (4E5) TA336918 - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

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  Western Blot: iNOS Antibody (4E5) TA336918 - Analysis using iNOS mAb against human iNOS (AA: 997-1058) recombinant protein. (Expected MW is 32.6 kDa)

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  Immunohistochemistry-Paraffin: iNOS Antibody (4E5) TA336918 - Analysis of paraffin-embedded liver cancer tissues using iNOS mouse mAb with DAB staining.

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  Flow Cytometry: iNOS Antibody (4E5) TA336918 - An intracellular stain was performed on A549 cells with TA336918PE (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 2.5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to phycoerythrin (PE).

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  Immunohistochemistry-Paraffin: iNOS Antibody (4E5) TA336918 - Analysis of paraffin-embedded breast cancer tissues using iNOS mouse mAb with DAB staining.

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  Western Blot: iNOS Antibody (4E5) TA336918 - Analysis using iNOS mouse mAb against Jurkat (1), Jurkat (2), A549 (3), HeLa (4), NIH3T3 (5) and MCF-7 (6) cell lysate.

• Flow Cytometry: iNOS Antibody (4E5) TA336918 - An intracellular stain was performed on A549 cells with TA336918APC (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated with antibody at 1 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to allophycocyanin (APC).

• Flow Cytometry: iNOS Antibody (4E5) - BSA Free TA336918 - An intracellular stain was performed on NIH3T3 cells with iNOS Antibody (4E5) TA336918 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

• Flow Cytometry: iNOS Antibody (4E5) - BSA Free TA336918 - An intracellular stain was performed on Caco-2 cells with iNOS Antibody (4E5) TA336918 (blue) and a matched isotype control MAB002 (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 1 ug/mL for 30 minutes at room temperature, followed by Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Dylight 550 (84540, Thermo Fisher).

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Specifications

Product Data

• Clone Name: 4E5
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Immunohistochemistry-Frozen, Immunohistochemistry, Flow (Intracellular), Flow Cytometry: 1:200-1:400, Immunohistochemistry-Paraffin: 1:200-1:1000, ELISA: 1:10000, Western Blot: 1:500-1:2000
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human iNOS expressed in E. coli. [UniProt# P35228]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 131 kDa
• Gene Name: nitric oxide synthase 2
• Database Link:
  NP_000616
  Entrez Gene 18126 MouseEntrez Gene 4843 Human
  UniProt ID P35228
• Background: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase which is expressed in liver and is inducible by a combination of lipopolysaccharide and certain cytokines. Three related pseudogenes are located within the Smith-Magenis syndrome region on chromosome 17.
• Synonyms: HEP-NOS; INOS; NOS; NOS2A
• Note: This iNOS (4E5) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Flow Cytometry and ELISA.

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Amyotrophic lateral sclerosis (ALS), Arginine and proline metabolism, Calcium signaling pathway, Long-term depression, Metabolic pathways, Pathways in cancer, Small cell lung cancer