Myelin Basic Protein (MBP) Mouse Monoclonal Antibody [Clone ID: 2H9]

CAT#: TA336919

Mouse Monoclonal MBP Antibody (2H9)

Product Images

ELISA: Myelin Basic Protein Antibody (2H9) TA336919 - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng).

• 

  Flow Cytometry: Myelin Basic Protein Antibody (2H9) TA336919 - Flow cytometric analysis of HepG2 cells using Myelin Basic Protein mouse mAb (green) and negative control (red).

• 

  Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) TA336919 - Immunohistochemical analysis of paraffin-embedded brain tissues using Myelin Basic Protein mouse mAb with DAB staining.

• 

  Immunocytochemistry/Immunofluorescence: Myelin Basic Protein Antibody (2H9) TA336919 - Immunofluorescence analysis of MSCS cells using Myelin Basic Protein mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 phalloidin.

• 

  Western Blot: MBP Antibody (2H9) TA336919 - Total human brain lysate was separated by SDS-PAGE on a 12% gel and transferred to PVDF membrane. The membrane was then probed with anti-MBP antibody at 2 ug/mL and detected using an anti-mouse HRP labeled secondary antibody and chemiluminescent substrate.

• 

  Immunohistochemistry-Paraffin: Myelin Basic Protein Antibody (2H9) TA336919 - Immunohistochemical analysis of paraffin-embedded cerebellum tissues using Myelin Basic Protein mouse mAb with DAB staining.

Show all 6 images

Specifications

Product Data

• Clone Name: 2H9
• Applications: ELISA, FC, IHC, WB
• Recommended Dilution: Immunohistochemistry-Frozen, Immunohistochemistry, Flow Cytometry: 1:200-1:400, ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry-Paraffin: 1:200-1:1000
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human Myelin Basic Protein expressed in E. coli. [UniProt# P02686]
• Formulation: PBS, 0.03% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ammonium sulfate precipitation
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 33 kDa
• Gene Name: myelin basic protein
• Database Link:
  NP_001020252
  Entrez Gene 4155 Human
  UniProt ID P02686
• Background: The protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes.
• Synonyms: MGC99675
• Note: This Myelin Basic Protein (2H9) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence, Flow Cytometry and ELISA.