DNA PKcs (PRKDC) Mouse Monoclonal Antibody [Clone ID: 3H6]

CAT#: TA336925

Mouse Monoclonal DNA-PKcs Antibody (3H6)

Product Images

ELISA: DNA-PKcs Antibody (3H6) TA336925 - Red: Control Antigen (100ng), Purple: Antigen (10ng), Green: Antigen (50ng), Blue: Antigen (100ng).

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  Western Blot: DNA-PKcs Antibody (3H6) TA336925 - Analysis using DNA PKcs mAb against HEK293 (1) and DNA PKcs(AA: 2638-2971)-hIgGFc transfected HEK293 (2) cell lysates.

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  Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) TA336925 - Analysis of paraffin-embedded lung cancer (left) and brain tissues (right) using DNA PKcs mouse mAb with DAB staining.

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  Immunocytochemistry/Immunofluorescence: DNA-PKcs Antibody (3H6) TA336925 - Analysis of Hela cells using DNA PKcs mouse mAb (green). Actin filaments have been labeled with Alexa Fluor-555 phalloidin (red).

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  Immunohistochemistry-Paraffin: DNA-PKcs Antibody (3H6) TA336925 - Analysis of paraffin-embedded breast cancer (left) and colon cancer (right) using DNA PKcs mouse mAb with DAB staining.

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  Western Blot: DNA-PKcs Antibody (3H6) TA336925 - HeLa (A), MCF7 (lB) and HEK293 (C) whole cell protein was separated by SDS-PAGE on 6% gel, transferred to PVDF and probed with TA336925. A single band of DNA PKcs reactivity at approximately 460 kDa was detected in all lysates.

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Specifications

Product Data

• Clone Name: 3H6
• Applications: ELISA, IHC, WB
• Recommended Dilution: ELISA: 1:10000, Immunocytochemistry/ Immunofluorescence: 1:200-1:1000, Immunohistochemistry-Paraffin: 1:200-1:1000, Western Blot: 1:500-1:2000, Immunohistochemistry
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: Purified recombinant fragment of human DNA PKcs expressed in E. coli. [UniProt# P78527]
• Formulation: Preservative: 0.05% Sodium Azide. Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Ascites
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 460 kDa
• Gene Name: protein kinase, DNA-activated, catalytic polypeptide
• Database Link:
  NP_001075109
  Entrez Gene 5591 Human
  UniProt ID P78527
• Background: The PRKDC gene encodes the catalytic subunit of a nuclear DNA-dependent serine/threonine protein kinase (DNA-PK). The second component is the autoimmune antigen Ku (MIM 152690), which is encoded by the G22P1 gene on chromosome 22q. On its own, the catalytic subunit of DNA-PK is inactive and relies on the G22P1 component to direct it to the DNA and trigger its kinase activity; PRKDC must be bound to DNA to express its catalytic properties.
• Synonyms: DNA-PKcs; DNAPK; DNPK1; HYRC; HYRC1; IMD26; p350; XRCC7
• Note: This DNA PKcs (3H6) antibody is useful for Western blot, Immunohistochemistry on paraffin-embedded sections, Immunocytochemistry/Immunofluorescence and ELISA. In WB, a band can be seen at 460-470 kDa representing DNA PKcs.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Cell cycle, Non-homologous end-joining