Sonic Hedgehog (SHH) Rabbit Polyclonal Antibody

CAT#: TA336926

Rabbit Polyclonal Sonic Hedgehog/Shh Antibody

Product Images

Western Blot: Sonic Hedgehog Antibody TA336926 - WB detection of Sonic Hedgehog in A. human stomach, B. human fetal liver membrane, C. PC12 and D. Cos7

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  Immunocytochemistry/Immunofluorescence: Sonic Hedgehog Antibody TA336926 - Sonic Hedgehog antibody was tested in SH-SY5Y cells with FITC (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Immunohistochemistry: Sonic Hedgehog Antibody TA336926 - IHC analysis of Sonic Hedgehog in mouse kidney.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Western Blot: 1-2 ug/ml, Immunohistochemistry-Paraffin: 1:200 - 1:400, Immunohistochemistry: 1:200 - 1:400, Immunocytochemistry/ Immunofluorescence: 1:200
• Reactivities: Human, Mouse, Rat, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to an N-terminal portion of the human SHH protein (between residues 1-75) [UniProt Q15465]
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: sonic hedgehog
• Database Link:
  NP_000184
  Entrez Gene 20423 MouseEntrez Gene 29499 RatEntrez Gene 6469 Human
  UniProt ID Q15465
• Background: Sonic Hedgehog (SHH), also known as HLP3, HPE3, MCOPCB5, SMMC1, and HHG1, is a gene that encodes for a protein instrumental in patterning the early embryo. Specifically, SHH has been implicated as the main signal in mapping out the development of the ventral neural tube, the anterior-posterior limb access, and the ventral somites. This occurs by SHH binding to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. SHH also regulates another target gene, the gli oncogene. In adults, SHH helps to control stem cell proliferation. The N-terminal form of SHH is associated with lipid rafts at the cell surface, while on a tissue level SHH is expressed in fetal intestine, liver, lung and kidney. Recent research has found SHH to modulate the spatial organization of neuroepithelial cell proliferation in the embryonic chick (PMID: 23031710), as well as detected a role for SHH in Idiopathic Pulmonary Fibrosis (PMID: 23023967).
• Synonyms: HHG1; HLP3; HPE3; MCOPCB5; SMMCI; TPT; TPTPS
• Note: This SHH antibody is useful for Western blot, Immunocytochemistry/Immunofluorescence, and Immunohistochemistry on paraffin embedded sections. In Western blot a band is observed at ~50 kDa. In Immunocytochemistry/Immunofluorescence cytoplasm/membrane staining was observed in SH-SY5Y cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Secreted Protein, Transmembrane
• Protein Pathways: Basal cell carcinoma, Hedgehog signaling pathway, Pathways in cancer