CD33 Mouse Monoclonal Antibody [Clone ID: 44M12D3]

CAT#: TA336936

Mouse Monoclonal Siglec-3/CD33 Antibody (44M12D3)

Product Images

Western Blot: Siglec-3/CD33 Antibody (44M12D3) TA336936 - Analysis of CD33 in A) a partial recombinant protein and B) HCT-116 lysate using CD33 antibody at 2 ug/ml. goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Immunohistochemistry-Paraffin: Siglec-3/CD33 Antibody (44M12D3) TA336936 - Human lung tissue stained with CD33 antibody (3 ug/ml), peroxidase-conjugate and DAB chromogen. Note cytoplasmic and membrane staining.

Specifications

Product Data

• Clone Name: 44M12D3
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:20-1:1000, Western Blot: 2-5 ug/ml, Immunohistochemistry-Paraffin: 3-6 ug/ml
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2a, kappa
• Clonality: Monoclonal
• Immunogen: Amino acids 42-159 of human CD33 were used as the immunogen for the antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: CD33 molecule
• Database Link:
  NP_001763
  Entrez Gene 945 Human
  UniProt ID P20138
• Synonyms: p67; SIGLEC-3; SIGLEC3
• Note: Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Hematopoietic cell lineage