EPCAM Mouse Monoclonal Antibody [Clone ID: 60N5D8]

CAT#: TA336940

Mouse Monoclonal EpCAM/CD326 Antibody (60N5D8)

Product Images

Western Blot: EpCAM/TROP1 Antibody (60N5D8) TA336940 - Analysis of EpCAM in HT-29 lysate in the (A) absence and (B) presence of immunizing peptide using EpCAM antibody at 2 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Flow Cytometry: EpCAM/TROP1 Antibody (60N5D8) TA336940 - Analysis of HT-29 cells using EpCAM antibody at 0.2 ug/10^6 cells. this antibody was used for this test. Top picture shows no cell surface staining seen, bottom shows intracellular staining of EpCAM (red represents EpCAM antibody and green represents isotype control).

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  Immunohistochemistry-Paraffin: EpCAM/TROP1 Antibody (60N5D8) TA336940 - FFPE human colon stained with EpCAM antibody (1:200), peroxidase-conjugate and DAB chromogen.

Specifications

Product Data

• Clone Name: 60N5D8
• Applications: FC, IHC, WB
• Recommended Dilution: Immunohistochemistry: 1:10-1:500, Western Blot: 2-5 ug/ml, Immunohistochemistry-Paraffin: 1:200-1:400, Flow Cytometry: 0.2-1 ug/10^6 cells
• Reactivities: Human
• Host: Mouse
• Isotype: IgG2b, kappa
• Clonality: Monoclonal
• Immunogen: Within the range of amino acids 289-335 were used as the immunogen for the EpCAM antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: epithelial cell adhesion molecule
• Database Link:
  NP_002345
  Entrez Gene 4072 Human
  UniProt ID P16422
• Synonyms: 4; DIAR5; EGP-2; EGP40; EGP314; ESA; HNPCC8; KS1; KSA; M4S1; MIC18; MK-1; TACSTD1; TROP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS, Transmembrane