NFkB Inducing Kinase NIK (MAP3K14) Rabbit Polyclonal Antibody

CAT#: TA336951

Rabbit Polyclonal NIK/MAP3K14 Antibody


USD 424.00

5 Days*

Size
    • 100 ul

Product Images

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Specifications

Product Data
Applications IHC, WB
Recommended Dilution Immunocytochemistry/ Immunofluorescence: 1:50 - 1:200, Western Blot: 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:400, Immunohistochemistry: 1:400
Reactivities Human, Mouse
Host Rabbit
Clonality Polyclonal
Immunogen A synthetic peptide made to a C-terminal portion of the human MAP3K14 protein (between residues 800-900) [UniProt Q99558]
Formulation PBS with 0.09% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Concentration lot specific
Purification Immunogen affinity purified
Conjugation Unconjugated
Storage Store at -20°C as received.
Stability Stable for 12 months from date of receipt.
Gene Name mitogen-activated protein kinase kinase kinase 14
Background MAP3K14, or NIK (Nuclear Factor-KB Inducing Kinase) is a serine/threonine protein kinase that activates the canonical and non-canonical NF-KB pathways to control transcription of proteins such as cytokines, chemokines and NF-KB signaling molecules. The NK-KB family of transcription factors is composed of a number of structurally related proteins that modulate an assortment of physiological processes from immune responses to cell death and survival. Regulation of NK-KB occurs by two distinct pathways; the canonical pathway mediated by the inhibitor of KB kinase complex, and the non-canonical pathway, involving p100 processing. In the canonical pathway NIK interacts and activates IKK alpha and IKK beta resulting in the release of RelA and p50 for gene transcription. In the non-canonical pathway, MAP3K14 (NIK) phosphorylates p100, resulting in its partial proteolysis to p52, which in turn mediates gene expression (PMID:20685151).
Synonyms FTDCR1B; HS; HSNIK; NIK
Note This MAP3K14 antibody detects a band at ~104 kDa in Western blot. In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in U2OS cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Reference Data
Protein Families Druggable Genome, Protein Kinase
Protein Pathways Apoptosis, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, T cell receptor signaling pathway

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