NFkB Inducing Kinase NIK (MAP3K14) Rabbit Polyclonal Antibody

CAT#: TA336951

Rabbit Polyclonal NIK/MAP3K14 Antibody

Product Images

Immunohistochemistry: NIK/MAP3K14 Antibody [NBP2-23603] - Analysis of MAP3K14 in mouse small intestine.

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  Immunocytochemistry/Immunofluorescence: NIK/MAP3K14 Antibody [NBP2-23603] - MAP3K14 antibody was tested in U2OS cells with DyLight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).

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  Western Blot: NIK/MAP3K14 Antibody [NBP2-23603] - Detection of MAP3K14 in U2OS cell lysate.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence: 1:50 - 1:200, Western Blot: 2.0 ug/ml, Immunohistochemistry-Paraffin: 1:400, Immunohistochemistry: 1:400
• Reactivities: Human, Mouse
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A synthetic peptide made to a C-terminal portion of the human MAP3K14 protein (between residues 800-900) [UniProt Q99558]
• Formulation: PBS with 0.09% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitogen-activated protein kinase kinase kinase 14
• Database Link:
  NP_003945
  Entrez Gene 53859 MouseEntrez Gene 9020 Human
  UniProt ID Q99558
• Background: MAP3K14, or NIK (Nuclear Factor-KB Inducing Kinase) is a serine/threonine protein kinase that activates the canonical and non-canonical NF-KB pathways to control transcription of proteins such as cytokines, chemokines and NF-KB signaling molecules. The NK-KB family of transcription factors is composed of a number of structurally related proteins that modulate an assortment of physiological processes from immune responses to cell death and survival. Regulation of NK-KB occurs by two distinct pathways; the canonical pathway mediated by the inhibitor of KB kinase complex, and the non-canonical pathway, involving p100 processing. In the canonical pathway NIK interacts and activates IKK alpha and IKK beta resulting in the release of RelA and p50 for gene transcription. In the non-canonical pathway, MAP3K14 (NIK) phosphorylates p100, resulting in its partial proteolysis to p52, which in turn mediates gene expression (PMID:20685151).
• Synonyms: FTDCR1B; HS; HSNIK; NIK
• Note: This MAP3K14 antibody detects a band at ~104 kDa in Western blot. In Immunocytochemistry/Immunofluorescence, cytoplasmic and nuclear staining was observed in U2OS cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Apoptosis, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, T cell receptor signaling pathway