ROR gamma (RORC) Rabbit Polyclonal Antibody

CAT#: TA336988

Rabbit Polyclonal ROR gamma/RORC/NR1F3 Antibody

Product Images

Western Blot: ROR gamma/RORC/NR1F3 Antibody TA336988 - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using TA336988.

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  Immunocytochemistry/Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody TA336988 - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were incubated with anti-ROR gamma overnight at 4C and detected with an anti-rabbit Dylight 488 (Green). Alpha tubulin (DM1A) NB100-690 was used as a co-stain and detected with an anti-mouse Dylight 550 (Red). Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Western Blot: ROR gamma/RORC/NR1F3 Antibody TA336988 - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/ml anti-ROR Gamma in 5% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

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  Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody TA336988 - Analysis of human testis using TA336988.

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  Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody TA336988 - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin. This antibody generated mainly a nuclear staining in a subset of cells and a weak to cytoplasmic staining was also observed in some cells.

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Western Blot: 0.5-2 ug/ml, Immunohistochemistry-Paraffin: 10 ug/ml, Immunohistochemistry: 2.5-10 ug/ml
• Reactivities: Human, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 1-50 of human ROR gamma was used as the immunogen.
• Formulation: PBS, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAR related orphan receptor C
• Database Link:
  NP_005051
  Entrez Gene 6097 Human
  UniProt ID P51449
• Synonyms: NR1F3; RORG; RZR-GAMMA; RZRG; TOR
• Note: Use in Immunohistochemistry and Immunocytochemistry/immunofluorescence reported in scientific literature (PMID 24317395)

Reference Data

• Protein Families: Druggable Genome, Transcription Factors