NF-kB p65 (RELA) Rabbit Polyclonal Antibody

CAT#: TA336998

Rabbit Polyclonal NFkB p65 NLS Antibody

Product Images

Western Blot: RelA/NFkB p65 NLS Antibody TA336998 - NFkB p65 NLS Antibody TA336998 - NFkB p65 NLS Antibody analysis of p65 NLS in Jurkat lysate using p65 NLS antibody at 1 ug/ml. Goat anti-rabbit Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Immunocytochemistry/Immunofluorescence: RelA/NFkB p65 NLS Antibody TA336998 - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-RelA/NFkB p65 NLS Antibody at 5 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.

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  Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody TA336998 - NFkB p65 NLS Antibody analysis of p65 NLS in formalin-fixed, paraffin-embedded human HeLa cells (top left) and LPS-stimulated HeLa cells (bottom left, right) using p65 NLS antibody at 5 ug/ml.

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  Immunohistochemistry-Paraffin: RelA/NFkB p65 NLS Antibody TA336998 - NFkB p65 NLS Antibody Formalin-fixed, paraffin-embedded human liver stained with p65 NLS antibody at 10 ug/ml.Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Immunocytochemistry/ Immunofluorescence: 5 ug/ml, Western Blot: 1-3 ug/ml, Immunohistochemistry-Paraffin: 10 ug/ml
• Reactivities: Human, Mouse, Bovine, Equine, Primate
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of the NFkB p65 NLS nuclear localization signal (NLS) (amino acids DTDDRHRIEEKRKRKT) was used as the immunogen for this antibody.
• Formulation: PBS, 30% glycerol, 0.1% Sodium Azide. Store at -20C. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Immunogen affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RELA proto-oncogene, NF-kB subunit
• Database Link:
  NP_068810
  Entrez Gene 19697 MouseEntrez Gene 5970 Human
  UniProt ID Q04206
• Synonyms: NFKB3; p65

Reference Data

• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Adipocytokine signaling pathway, Apoptosis, B cell receptor signaling pathway, Chemokine signaling pathway, Chronic myeloid leukemia, Cytosolic DNA-sensing pathway, Epithelial cell signaling in Helicobacter pylori infection, MAPK signaling pathway, Neurotrophin signaling pathway, NOD-like receptor signaling pathway, Pancreatic cancer, Pathways in cancer, Prostate cancer, RIG-I-like receptor signaling pathway, Small cell lung cancer, T cell receptor signaling pathway, Toll-like receptor signaling pathway