14-3-3 sigma (SFN) Rabbit Polyclonal Antibody

CAT#: TA337133

Rabbit Polyclonal 14-3-3 sigma/Stratifin Antibody

Product Images

Western Blot: 14-3-3 sigma/Stratifin Antibody TA337133 - analysis of 14-3-3 sigma (248 amino acid isoform) using 14-3-3 sigma polyclonal antibody. Human A431 cell lysate in the 1) absence and 2) presence of immunizing peptide, and 3) HeLa cell lysate probed with 14-3-3 sigma antibody at 3 ug/ml.

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  Western Blot: 14-3-3 sigma/Stratifin Antibody TA337133 - Analysis of 14-3-3 sigma (169 amino acid isoform) using 14-3-3 sigma antibody. Human brain tissue in the 1) absence and 2) presence of immunizing peptide, 3) mouse brain and 4) rat brain tissue probed with 14-3-3 sigma antibody at 3 ug/ml.

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  Immunohistochemistry-Paraffin: 14-3-3 sigma/Stratifin Antibody TA337133 - Formalin-fixed, paraffin-embedded human tonsil (squamous epithelium) probed with 14-3-3 sigma antibody at 10 ug/ml.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Knockdown Validated, Western Blot: 3-5ug/ml~, Immunohistochemistry-Paraffin: 5-10ug/ml
• Reactivities: Human, Amphibian, Bovine, Canine, Equine, Opossum
• Host: Rabbit
• Clonality: Polyclonal
• Immunogen: A portion of amino acids 120-170 of human 14-3-3 sigma was used as the immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein A purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: stratifin
• Database Link:
  NP_006133
  Entrez Gene 2810 Human
  UniProt ID P31947
• Background: 14-3-3 sigma (stratifin, SFN, epithelial cell marker protein 1) is a member of a highly conserved family of 14-3-3 proteins that are present in all eukaryotic organisms. There are 7 known mammalian 14-3-3 isoforms (sigma epsilon, beta, zeta, gamma, theta, tau and they play important roles in many biological activities by binding to and altering the subcellular localization and/or stability of key molecules in various signaling cascades. 14-3-3 sigma was originally characterized as a human mammary epithelium marker and later rediscovered as an important molecule for cell cycle checkpoint regulation. Recently it has been reported that 14-3-3 sigma may serve as a prognosis marker predicting survival of pancreatic cancer patient treatment. 14-3-3 sigma is the only 14-3-3 isoform induced by the tumor suppressor protein p53, in response to irradiation and other DNA-damaging agents. 14-3-3 sigma is a p53-regulated inhibitor of G2/M progression and acts as a tumor suppressor gene that is inactivated by methylation of its 5' CpG islands in epithelial tumor cells. Two isoforms of human 14-3-3 sigma are produced by alternative splicing.
• Synonyms: YWHAS

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Cell cycle, p53 signaling pathway