AGR2 Mouse Monoclonal Antibody [Clone ID: 10E2]

CAT#: TA337147

Mouse Monoclonal AG-2 Antibody (10E2)

Product Images

Western Blot: AG-2 Antibody (10E2) TA337147 - Analysis of human stomach in the A) absense and B) presence of immunizing peptide, C) mouse stomach, D) rat stomach tissue lysate, and E) HCT-116 cell lysate using AGR2 antibody at 5 ug/ml. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

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  Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) TA337147 - Formalin-fixed, paraffin-embedded human spleen tissue stained with 5 ug/ml concentration of AGR2 antibody .

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  Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) TA337147 - Paraffin-embedded human small intestine tissue stained with AGR2 antibody at 10 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) TA337147 - Formalin-fixed, paraffin-embedded human breast stained with AGR2 antibody at 5 ug/ml. Staining of formalin-fixed tissues is enhanced by boiling tissue sections in 10 mM sodium citrate buffer, pH 6.0 for 10-20 min followed by cooling at RT for 20 min.

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  Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) TA337147 - IHC-P detection of AGR2 protein in a section of human bladded cancer using 5 ug/ml concentration of AGR2 antibody. The AGR2 immunoreactivity was observed in cellular cytoplasm and the inter-cellular spaces in cancerous areas of the sections. [40X magnification]

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  Immunohistochemistry-Paraffin: AG-2 Antibody (10E2) TA337147 - IHC-P detection of AGR2 protein in a human ovarian cancer section using 5 ug/ml concentration of AGR2 antibody. The representative image shows AGR2 immuno-reactivity in the developing ovarian cancer and the follicle cells of Graafian follicles. The cells of medulla region in the ovary did not show AGR2 positivity. [10X magnification]

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Specifications

Product Data

• Clone Name: 10E2
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry: 5 ug/ml, Western Blot: 4-6ug/ml, Immunohistochemistry-Paraffin: 5 ug/ml
• Reactivities: Human, Mouse, Bovine, Canine, Equine, Primate
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: A portion of amino acids 50-100 of human AGR2 was used as the immunogen for the antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: anterior gradient 2, protein disulphide isomerase family member
• Database Link:
  NP_006399
  Entrez Gene 23795 MouseEntrez Gene 10551 Human
  UniProt ID O95994
• Background: hAG-2 and hAG-3 are recently discovered human homologues of the secreted Xenopus laevis proteins XAG-1/2 (AGR1 and AGR2) that are expressed in the cement gland, an ectodermal organ in the head associated with anteroposterior fate determination during early development. Although the roles of hAG-2 and hAG-3 in mammalian cells are unknown, both proteins share a high degree of protein sequence homology and lie adjacent to one another on chromosome 7p21.
• Synonyms: AG2; GOB-4; HAG-2; HEL-S-116; PDIA17; XAG-2

Reference Data

• Protein Families: Secreted Protein