IL22 Mouse Monoclonal Antibody [Clone ID: 8F11E2]

CAT#: TA337159

Mouse Monoclonal IL-22 Antibody (8F11E2)

Product Images

Immunohistochemistry-Paraffin: IL-22 Antibody (8F11E2) TA337159 - Analysis of a FFPE tissue section of human tonsil using 5 ug/ml dilution of IL-22 (8F11E2) antibody. The staining was developed using HRP labeled anti-mouse secondary antibody and DAB reagent, and nuclei of cells were counter-stained with hematoxylin.

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  Western Blot: IL-22 Antibody (8F11E2) TA337159 - Analysis of IL-22 in A) recombinant protein and B) human spleen lysate using IL-22 antibody at 1 ug/mL. Goat anti-mouse Ig HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Specifications

Product Data

• Clone Name: 8F11E2
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry, Western Blot: 1 - 3 ug/mL, Immunohistochemistry-Paraffin: 5 ug/mL
• Reactivities: Human
• Host: Mouse
• Clonality: Monoclonal
• Immunogen: MAPISSHCRL DkSNFQQPYI TNRTFMLAkE ASLADNNTDV RLIGEkLFHG VSMSERCYLM kQVLNFTLEE VLFPQSDRFQ PYMQEVVPFL ARLSNRLSTC HIEGDDLHIQ RNVQkLkDTV kkLGESGEIk AIGELDLLFM SLRNACI
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: interleukin 22
• Database Link:
  NP_065386
  Entrez Gene 50616 Human
  UniProt ID Q9GZX6
• Background: IL-22 is an inflammatory cytokine produced by so-called Th22 cells, especially, as well as Th17 cells. Activating through STAT3 signaling cascades, IL-22 is also expressed by numerous types of lymphocytes including LTi and LTi-like cells in the gut, Nk and T cells, and Langerhans cells which are APCs in the skin (1, 2). The protein is therefore expressed in effector cells of both the Innate and Adaptive Immune systems. Th22 cells, a subset of CD4 cells, preferentially express IL-22 and also express CCR6, CCR4 and CCR10--the latter two representing skin-homing receptors (3-5). Th22 cells do not express RORt or T-bet IL-17A or IFN (4). However, AHR appears to be a key priming transcription element for IL-22 as for IL-17A expression (5). IL-6 and TNF induce Th22 priming (1, 5). It also appears to have positive activities in wound healing and tissue repair since deficiencies in IL-22 in mouse models delay healing of intestinal biopsies (6). Other tissue repair activities have also been identified with the protein. Taken together, IL-22 is thought to be a key to maintaining a balance between inflammation and tolerance, or homeostasis, especially in skin and mucosal organs like the gut.
• Synonyms: IL-21; IL-22; IL-D110; IL-TIF; ILTIF; TIFa; TIFIL-23; zcyto18

Reference Data

• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• Protein Pathways: Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway