EBI3 Mouse Monoclonal Antibody [Clone ID: 15k8D10]

CAT#: TA337166

Mouse Monoclonal IL-27/IL-35 EBI3 Subunit Antibody (15k8D10)

Product Images

Immunohistochemistry-Paraffin: EBI3 Antibody (15k8D10) TA337166 - Formalin-fixed, paraffin-embedded human spleen (left) and lymph node metastatic malignant melanoma (right) stained with IL-35 antibody (5 ug/ml) using peroxidase-conjugate and DAB chromogen.

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  Western Blot: EBI3 Antibody (15k8D10) TA337166 - Analysis of IL-35-Fc protein (A) and human Fc (B) using IL-35 antibody at 4ug/ml.

Specifications

Product Data

• Clone Name: 15k8D10
• Applications: IHC, WB
• Recommended Dilution: Immunohistochemistry-Paraffin: 5ug/ml, Western Blot: 3-5ug/ml~, Tissue Culture Substratum, Immunohistochemistry, Immunocytochemistry/ Immunofluorescence
• Reactivities: Human
• Host: Mouse
• Isotype: IgG1, kappa
• Clonality: Monoclonal
• Immunogen: A full-length human Interleukin-35-Fc fusion protein was used as immunogen for this antibody.
• Formulation: PBS containing 0.05% BSA, 0.05% Sodium Azide. Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Concentration: lot specific
• Purification: Protein G purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: Epstein-Barr virus induced 3
• Database Link:
  NP_005746
  Entrez Gene 10148 Human
  UniProt ID Q14213
• Background: IL-35 is a novel heterodimeric cytokine composed of the IL-12p35 subunit and the Epstein-Barr virus-induced gene 3 (EBI3) protein. As one of the IL-12 family members, IL-35 is known to play an essential role in immune regulation of the CD4+CD25+ regulatory T (Treg) cells, from which IL-35 is also specifically produced. IL-35 is able to expand CD4+CD25+ Treg cells whereas it can inhibit proliferation of CD4+CD25- effector T (Teff) cells and development of proinflammatory Th17 cells, suppressing inflammatory responses.
• Synonyms: IL-27B; IL27B

Reference Data

• Protein Families: Druggable Genome, Secreted Protein