DDX3 (DDX3X) Rabbit Polyclonal Antibody

CAT#: TA341555

Rabbit Polyclonal Anti-DDX3X Antibody

Product Images

Sample Type: 1. Human NT-2 cells (60ug); 2. mouse brain extracts (80ug); Primary Antibody Dilution: 2 ug/ml; Secondary Antibody: IRDye 800CW goat anti-rabbit from Li-COR Bioscience; Secondary Antibody Dilution: 1: 20,000; Image Submitted by: Yuzhi Chen; U

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  WB Suggested Anti-DDX3X Antibody Titration: 0.2-1 ug/ml; ELISA Titer: 1:62500; Positive Control: THP-1 cell lysate

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  Sample Type. Human brain stem cells (NT2) Primary Antibody Dilution. 1:500Secondary Antibody. Goat anti-rabbit Alexa Fluor 594Secondary Antibody Dilution. 1:1000Color/Signal Descriptions. Red: DDX3X Blue: DAPIGene Name. DDX3XSubmitted by. Dr. Yuzhi

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: IHC, WB
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-DDX3X antibody: synthetic peptide directed towards the N terminal of human DDX3X. Synthetic peptide located within the following region: MSHVAVENALGLDQQFAGLDLNSSDNQSGGSTASKGRYIPPHLRNREATK
• Formulation: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
  Note that this product is shipped as lyophilized powder to China customers.
• Concentration: lot specific
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 73 kDa
• Gene Name: DEAD-box helicase 3, X-linked
• Database Link:
  NP_001347
  Entrez Gene 13205 MouseEntrez Gene 1654 Human
  UniProt ID O00571
• Background: DEAD box proteins, characterized byThe conserved motif Asp-Glu-Ala-Asp (DEAD), are putative RNA helicases.They are implicated in a number of cellular processes involving alteration of RNA secondary structure such as translation initiation, nuclear and mitochondrial splicing, and ribosome and spliceosome assembly. Based onTheir distribution patterns, some members ofThis family are believed to be involved in embryogenesis, spermatogenesis, and cellular growth and division.This gene encodes a DEAD box protein, which interacts specifically with hepatitis C virus core protein resulting a change in intracellular location.This gene has a homolog located inThe nonrecombining region ofThe Y chromosome.The protein sequence is 91% identical betweenThis gene andThe Y-linked homolog. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jul 2010]
• Synonyms: CAP-Rf; DBX; DDX3; DDX14; HLP2
• Note: Immunogen Sequence Homology: Dog: 100%; Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Bovine: 100%; Rat: 93%; Rabbit: 93%; Guinea pig: 93%

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: RIG-I-like receptor signaling pathway