Histone H2A.X (H2AFX) Mouse Monoclonal Antibody [Clone ID: 3E11-1H6-G3]

CAT#: TA346959

Mouse Monoclonal Phospho-Histone H2A.X (Ser139) Antibody

Product Images

Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.

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  Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).

Specifications

Product Data

• Clone Name: 3E11-1H6-G3
• Applications: IF, WB
• Recommended Dilution: WB: 1:2000, IF: 1:400
• Reactivities: Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The immunogen for H2AX(Pi-Ser139) antibody: Synthetic phosphopeptide corresponding to residues surrounding Ser139 of human H2A.X.
• Formulation: Purified mouse monoclonal antibody in PBS(pH 7.4) containing with 0.03% Proclin300 and 50% glycerol.
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: H2A histone family member X
• Database Link:
  NP_002096
  Entrez Gene 15270 Mouse
  UniProt ID P16104
• Background: Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene encodes a member of the histone H2A family, and generates two transcripts through the use of the conserved stem-loop termination motif, and the polyA addition motif.
• Synonyms: H2A; H2A.X; H2AX; X

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Systemic lupus erythematosus