PDHA1 Mouse Monoclonal Antibody [Clone ID: 3H2-F8-B5]

CAT#: TA347015

Mouse Monoclonal pyruvate dehydrogenase (lipoamide) alpha 1 Antibody

Specifications

Product Data

• Clone Name: 3H2-F8-B5
• Applications: IF, WB
• Recommended Dilution: WB: 1:1000, IF: 1:100
• Reactivities: Human, Mouse
• Host: Mouse
• Isotype: IgG1
• Clonality: Monoclonal
• Immunogen: The immunogen for PDHA1 antibody: purified recombinant human Pyruvate Dehydrogenase protein fragments expressed in E.coli.
• Formulation: Purified mouse monoclonal in buffer containing 0.1M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.02% sodium azide, 50%,glycerol
• Purification: Affinity purified
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 43 kDa
• Gene Name: pyruvate dehydrogenase (lipoamide) alpha 1
• Database Link:
  NP_000275
  Entrez Gene 18597 MouseEntrez Gene 5160 Human
  UniProt ID P08559
• Background: The pyruvate dehydrogenase (PDH) complex is a nuclear-encoded mitochondrial multienzyme complex that catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2), and provides the primary link between glycolysis and the tricarboxylic acid (TCA) cycle. The PDH complex is composed of multiple copies of three enzymatic components: pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydrogenase (E3). The E1 enzyme is a heterotetramer of two alpha and two beta subunits. This gene encodes the E1 alpha 1 subunit containing the E1 active site, and plays a key role in the function of the PDH complex. Mutations in this gene are associated with pyruvate dehydrogenase E1-alpha deficiency and X-linked Leigh syndrome. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: PDHA; PDHAD; PDHCE1A; PHE1A

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Metabolic pathways, Pyruvate metabolism, Valine, leucine and isoleucine biosynthesis