AGO1 Rabbit Polyclonal Antibody

CAT#: TA347077

Rabbit Polyclonal EF2C1/2 Antibody

Product Images

WB using the antibody against IEF2C1/2 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 97 kDa); the marker (in kDa) is shown on the left.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human IEF2C1/2. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3, 500.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100); Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-EF2C1/2 antibody: human IEF2C1/2 (eukaryotic translation initiation factor 2C1 and 2), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of both EIF2C1 and EIF2C2.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: argonaute 1, RISC catalytic component
• Database Link:
  NP_036331
  Entrez Gene 26523 Human
  UniProt ID Q9UL18
• Background: IEF2C1 and 2 (UniProtKB/Swiss-Prot entries Q9UL18 and Q9UKV8) are members of the Argonaute family. These proteins are involved in short-interfering-RNA-mediated gene silencing. This process is mediated by the RISC (RNA induced silencing complex), a multicomponent nuclease that is able to degrade double stranded RNAs. The mRNA degradation is triggered by the association of specific siRNAs with their homologous mRNAs. IEF2C2 has been shown to be essential for embryonic development.
• Synonyms: EIF2C; EIF2C1; GERP95; Q99

Reference Data

• Protein Families: Druggable Genome