ASH2L Rabbit Polyclonal Antibody

CAT#: TA347080

Rabbit Polyclonal ASH2 Antibody

Product Images

WB was performed using the hASH2 ab at 1:1,000 in TBS-Tween with 5% skimmed milk on nuclear extracts from HeLa cells (upper panel: lane 1) and ectopically expressed FLAG-tagged ASH2 (ASH2-F, upper panel: lane 2). The lower panel shows the same WB was performed using nuclear extracts from HeLa (HeLa NE, 20 ug) with the ASH2 ab at 1:1,000 in TBS-Tween with 5% skimmed milk. The molecular weight marker (in kDa) is shown on the left; the location of the protein of interest is indicated on the right.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-ASH2 antibody: human Ash2 (absent, small, or homeotic 2), using a recombinant protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: ASH2 like histone lysine methyltransferase complex subunit
• Database Link:
  NP_004665
  Entrez Gene 9070 Human
  UniProt ID Q9UBL3
• Background: ASH2 (UniProtKB/Swiss-Prot entry Q9UBL3) is a component of the SET1/ASH2 histone methyltransferase (HMT) complex. This complex specifically methylates K4 of histone H3, thereby activating transcription. Methylation of K4 is blocked by premethylation of the neighboring K9, a repressor of transcription. This indicates that the SET1/ASH2 HMT complex mediates the crosstalk between K9 methylation and K4 methylation. ASH2 plays a role in hematopoiesis and may be associated with some kinds of leukemia.
• Synonyms: ASH2; ASH2L1; ASH2L2; Bre2

Reference Data

• Protein Families: Druggable Genome, Transcription Factors