HP1 alpha (CBX5) Rabbit Polyclonal Antibody

CAT#: TA347091

Rabbit Polyclonal HP1alpha/beta/gamma Antibody

Product Images

WB was performed on nuclear extracts from HeLa cells (20 ug) with the antibody against human HP1a, β and ? diluted 1:1,000 in TBS-Tween containing 5% skimmed milk (Figure 1). The molecular weight marker (in kDa) is shown on the left; the expected location of HP1a, HP1β and HP1? is indicated on the right.

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  HeLa cells were stained with the antibody against HP1a, β, ? and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the HP1a, β, ? antibody (left) diluted 1:500 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

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  ChIP assays were performed using NIH3T3 cells and 4 ug of the antibody against HP1a, β and ?. QPCR was performed on the IP'd DNA with optimized primer sets for the rDNA promoter and for a subtelomeric sequence of chromosome 19. Image shows the relative enrichment as compared to a no antibody negative control ChIP.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: ChIP (4ug/ChIP); Western blotting (1:1,000); IF (1:500)
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-HP1.,.,. antibody: human HP1 . (Heterochromatin protein 1 homolog beta), using the full length recombinant GST tagged protein. The antibody also recognizes the . and . isoforms.
• Concentration: lot specific
• Purification: Protein G purified polyclonal antibody in PBS containing 0.05% azide and 0.05% ProClin 300.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: chromobox 5
• Database Link:
  NP_036249
  Entrez Gene 12419 MouseEntrez Gene 23468 Human
  UniProt ID P45973
• Background: HP1 alpha, beta and gamma (UniProt/Swiss-Prot entry P45973, P83916 and Q13185) are components of heterochromatin. They recognize and bind histone H3 tails methylated at 'Lys-9', leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the inner nuclear membrane.
• Synonyms: HEL25; HP1; HP1A