KDM4B Rabbit Polyclonal Antibody

CAT#: TA347227

Rabbit Polyclonal JMJD2b Antibody

Product Images

WB using the antibody against JMJD2b diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right; the marker (in kDa) is shown on the left.

• 

  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human JMJD2b. The wells were coated with the peptide used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:3,000.

• 

  HeLa cells were stained with the antibody against JMJD2b and with DAPI. Cells were fixed with 4% formaldehyde for 10' and blocked with PBS/TX-100 containing 5% normal goat serum and 1% BSA. The cells were immunofluorescently labelled with the JMJD2b antibody (left) diluted 1:200 in blocking solution followed by an anti-rabbit antibody conjugated to Alexa488. The middle panel shows staining of the nuclei with DAPI. A merge of the two stainings is shown on the right.

Specifications

Product Data

• Applications: ELISA, IF, WB
• Recommended Dilution: ELISA (1:200); Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-JMJD2b antibody: human JMJD2b (Jumonji Domain containing 2b), using a KLH-conjugated synthetic peptide containing an amino acid sequence from the central part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: lysine demethylase 4B
• Database Link:
  NP_055830
  Entrez Gene 23030 Human
  UniProt ID O94953
• Background: JMJD2b (UniProtKB/Swiss-Prot entry O94953), belongs to the JMJD2 family of histone demethylases which play an important role in the establishment of the histone code. JMJD2b specifically demethylates the trimethylated K9 of histone H3. It is not able to demethylate K4, K27 and K36 of histone H3, nor K20 of histone H4.
• Synonyms: JMJD2B; TDRD14B

Reference Data

• Protein Families: Druggable Genome