MBD3 Rabbit Polyclonal Antibody

CAT#: TA347241

Rabbit Polyclonal MBD3 Antibody

Product Images

WB on nuclear extracts from a Human leukemic monocyte lymphoma cell line (U937 NE, 60 ug) with the ab at 1:2,000 in TBS-Tween with 5% milk. B: TY1-tagged MBD3 (TTE-MBD3) was over-expressed in osteosarcoma cells (U2OS). Nuclear extracts were prepared and separated by electrophoresis. An ab against the TY1 tag or the ab against MBD3 (1:2,000 in TBS-Tween & 5% milk) were used to detect TTE-MBD3 (lane 1 and 2, respectively).

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  Determination of the antibody titer.To determine the titer of the antibody, an ELISA was performed using a serial dilution of the antibody against human MBD3. The wells were coated with the peptides used for immunisation of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:13, 890.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100 ?? 1:500); Western blotting (1:2,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-MBD3 antibody: human MBD3 (Methyl-CpG-binding domain protein 3), using three different KLH-conjugated synthetic peptides.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: methyl-CpG binding domain protein 3
• Database Link:
  NP_001268382
  Entrez Gene 53615 Human
  UniProt ID O95983
• Background: MBD3 (UniProtKB/Swiss-Prot entry O95983) is a transcriptional repressor which plays a role in gene silencing. Surprisingly, MBD3 does not seem to bind methylated DNA by itself, but acts by the recruitment of histone deacetylases and DNA methyltransferases. MBD3 also forms a heterodimer with MBD2 and is part of the NuRD and the MeCP1 complex. Further, MBD3 interacts with HDAC1, MTA2, DNMT1, p66-alpha and p66-beta.
• Synonyms: MBD3

Reference Data

• Protein Families: Druggable Genome, Transcription Factors