Poly(A) RNA polymerase, mitochondrial (MTPAP) Rabbit Polyclonal Antibody

CAT#: TA347250

Rabbit Polyclonal PAPD1 Antibody

Product Images

WB using the antibody against PAPD1 diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 66 kDa); the marker (in kDa) is shown on the left.

• 

  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human PAPD1. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:47,000.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:500) ; Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-PAPD1 antibody: human PAPD1 (PAP associated domain containing 1), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: mitochondrial poly(A) polymerase
• Database Link:
  NP_060579
  Entrez Gene 55149 Human
  UniProt ID Q9NVV4
• Background: PAPD1 (UniProtKB/Swiss-Prot entry Q9NVV4) is a mitochondrial RNA polymerase that generates the poly(A) tail of mitochondrial RNA's by adding AMP monomers to the 3'-end. PAPD1 might also be responsible for the introduction of UAA stop codons, which are not encoded by the mitochondrial DNA, in some RNA molecules.
• Synonyms: PAPD1; SPAX4