PAPOLA Rabbit Polyclonal Antibody

CAT#: TA347261

Rabbit Polyclonal PAPOLA Antibody

Product Images

WB using the antibody against PAPOLA diluted 1:1,000 in TBS-Tween containing 5% skimmed milk. The position of the protein of interest is indicated on the right (expected size: 83 kDa); the marker (in kDa) is shown on the left.

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  Determination of the titer To determine the titer, an ELISA was performed using a serial dilution of the antibody against human PAPOLA. The plates were coated with the peptide used for immunization of the rabbit. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:22, 500.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100 ?? 1:500); Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-PAPOLA antibody: human PAPOLA (polyA polymerase alpha), using a KLH-conjugated synthetic peptide containing a sequence from the C-terminal part of the protein.
• Concentration: lot specific
• Purification: Whole antiserum from rabbit containing 0.05% azide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: poly(A) polymerase alpha
• Database Link:
  NP_116021
  Entrez Gene 10914 Human
  UniProt ID P51003
• Background: PAPOLA (UniProtKB/Swiss-Prot entry P51003) is an RNA polymerase that generates the poly(A) tail of RNA's by adding AMP monomers to the 3'-end. PAPOLA may also be required for the endoribonucleolytic cleavage reaction at some polyadenylation sites.
• Synonyms: PAP

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: RNA degradation