RUNX1T1 Rabbit Polyclonal Antibody

CAT#: TA347281

Rabbit Polyclonal AML-ETO Antibody

Product Images

WB was performed on seven GST-fusion proteins containing different fragments of AML-ETO (1-7) with the ab at 1:1,000. The antibody raised against AML-ETO recognizes the ETO part (lane 2) of the fusion protein. C: WB was performed on nuclear extracts from KAS-6/1 cells (human myeloma cell line) and SKNO-1 cells (human acute myeloblastic leukaemia) with the ab against AML-ETO at 1:1,000. The location of AML-ETO and a presumed splice variant (missing 106 C-terminal amino acids) are indicated.

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  Determination of the antibody titer To determine the titer of the antibody, an ELISA was performed using a serial dilution of antibody against AML-ETO, crude serum and flow through in antigen coated wells. By plotting the absorbance against the antibody dilution (Figure 1), the titer of the antibody was estimated to be 1:5, 250.

Specifications

Product Data

• Applications: ELISA, WB
• Recommended Dilution: ELISA (1:100); Western blotting (1:1,000)
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for anti-AML-ETO antibody: the AML-ETO (RUNX1) fusion protein, using 3 different KLH-conjugated synthetic peptides. The antibody recognizes the ETO (RUNX1T1) part of the fusion protein.
• Concentration: lot specific
• Purification: Store at -20°C; for long storage, store at -80°C. Avoid multiple freeze-thaw cycles.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RUNX1 translocation partner 1
• Database Link:
  NP_783553
  Entrez Gene 862 Human
  UniProt ID Q06455
• Background: RUNX1T1 (UniProt/Swiss-Prot entry Q06455) is a putative transcription factor which forms a heterodimer with CBFA2T3. Defects in RUNX1T1 have been associated with acute myeloid leukemia (AML-M2) and may be a cause of colorectal cancer.
• Synonyms: AML1T1; CBFA2T1; CDR; ETO; MTG8; ZMYND2

Reference Data

• Protein Families: Transcription Factors
• Protein Pathways: Acute myeloid leukemia, Pathways in cancer