RAN Goat Polyclonal Antibody

CAT#: TA348979

Goat Polyclonal Anti-RAN (aa 197-210) Antibody

Product Images

TA348979 (1 ug/ml) staining of Human Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA348979 (0.1 ug/ml) staining of Mouse (A) and Rat (B) Testis lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.1-1 ug/ml
• Reactivities: Human, Mouse, Rat (Expected from sequence similarity: Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for Anti-RAN (aa 197-210) Antibody: Peptide with sequence C-YEHDLEVAQTTALP, from the C Terminus of the protein sequence according to NP_006316.1.
• Formulation: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: RAN, member RAS oncogene family
• Database Link:
  NP_006316
  Entrez Gene 19384 MouseEntrez Gene 84509 RatEntrez Gene 5901 Human
  UniProt ID P62826
• Background: RAN (ras-related nuclear protein) is a small GTP binding protein belonging to the RAS superfamily that is essential for the translocation of RNA and proteins through the nuclear pore complex. The RAN protein is also involved in control of DNA synthesis and cell cycle progression. Nuclear localization of RAN requires the presence of regulator of chromosome condensation 1 (RCC1). Mutations in RAN disrupt DNA synthesis. Because of its many functions, it is likely that RAN interacts with several other proteins. RAN regulates formation and organization of the microtubule network independently of its role in the nucleus-cytosol exchange of macromolecules. RAN could be a key signaling molecule regulating microtubule polymerization during mitosis. RCC1 generates a high local concentration of RAN-GTP around chromatin which, in turn, induces the local nucleation of microtubules. RAN is an androgen receptor (AR) coactivator that binds differentially with different lengths of polyglutamine within the androgen receptor. Polyglutamine repeat expansion in the AR is linked to Kennedy's disease (X-linked spinal and bulbar muscular atrophy). RAN coactivation of the AR diminishes with polyglutamine expansion within the AR, and this weak coactivation may lead to partial androgen insensitivity during the development of Kennedy's disease. [provided by RefSeq, Jul 2008]
• Synonyms: ARA24; Gsp1; TC4

Reference Data

• Protein Families: Druggable Genome, Transcription Factors