Cardiac Troponin I (TNNI3) Goat Polyclonal Antibody

CAT#: TA348993

Goat Polyclonal Anti-cardiac troponin T Antibody

Product Images

TA348993 (0.03 ug/ml) staining of Rat Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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  TA348993 (0.01 ug/ml) staining of Pig Heart lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 0.03-0.1 ug/ml
• Reactivities: Human, Mouse, Rat, Pig (Expected from sequence similarity: Feline, Dog, Cow)
• Host: Goat
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: The immunogen for Anti-cardiac troponin T Antibody: Peptide with sequence C-RNRINDNQKVSKT, from the C Terminus of the protein sequence according to NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1.
• Formulation: Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: lot specific
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Gene Name: troponin I3, cardiac type
• Database Link:
  NP_000354
  Entrez Gene 21954 MouseEntrez Gene 29248 RatEntrez Gene 403566 DogEntrez Gene 7137 Human
  UniProt ID P19429
• Background: The protein encoded by this gene is the tropomyosin-binding subunit of the troponin complex, which is located on the thin filament of striated muscles and regulates muscle contraction in response to alterations in intracellular calcium ion concentration. Mutations in this gene have been associated with familial hypertrophic cardiomyopathy as well as with dilated cardiomyopathy. Transcripts for this gene undergo alternative splicing that results in many tissue-specific isoforms, however, the full-length nature of some of these variants has not yet been determined. [provided by RefSeq, Jul 2008]
• Synonyms: CMD1FF; CMD2A; CMH7; cTnI; RCM1; TNNC1
• Note: This antibody is expected to recognize all reported isoforms (NP_000355.2; NP_001001430.1; NP_001001431.1; NP_001001432.1).

Reference Data

• Protein Families: Druggable Genome, ES Cell Differentiation/IPS, Stem cell - Pluripotency
• Protein Pathways: Cardiac muscle contraction, Dilated cardiomyopathy, Hypertrophic cardiomyopathy (HCM)