IL12A Rabbit Polyclonal Antibody

CAT#: TA349020

Rabbit Polyclonal Anti-IL-12A Antibody

Product Images

Western blot analysis of IL-12A in HeLa cell lysate with IL-12A antibody at 0.5ug/ml in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of IL-12A in human pancreas tissue with IL-12A Antibody at 5ug/ml.

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  Immunofluorescence of IL-12A in human pancreas tissue with IL-12A Antibody at 20ug/ml.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: IL-12A antibody was raised against an 18 amino acid peptide near the carboxy terminus of human IL-12A. The immunogen is located within the last 50 amino acids of IL-12A.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: IL-12A antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 22 kDa; Observed: 22 kDa
• Gene Name: interleukin 12A
• Database Link:
  NP_000873
  Entrez Gene 3592 Human
  UniProt ID P29459
• Background: IL-12A is subunit of the cytokine IL-12 which acts on T- and natural killer cells, and has a broad array of biological activities. IL-12 is a disulfide-linked heterodimer composed of the 35-kD subunit and a 40-kD subunit that is a member of the cytokine receptor family (1). IL-12 is required for the T-cell-independent induction of interferon (IFN)-gamma, and is important for the differentiation of both Th1 and Th2 cells (1,2). The responses of lymphocytes to IL-12 are mediated by the activator of transcription protein STAT4 (2). Nitric oxide synthase 2A (NOS2A/NOS2) is found to be required for the signaling process of this cytokine in innate immunity (3).
• Synonyms: CLMF; IL-12A; NFSK; NKSF1; P35

Reference Data

• Protein Families: Druggable Genome, Secreted Protein
• Protein Pathways: Allograft rejection, Cytokine-cytokine receptor interaction, Jak-STAT signaling pathway, RIG-I-like receptor signaling pathway, Toll-like receptor signaling pathway, Type I diabetes mellitus