TRPC3 Rabbit Polyclonal Antibody

CAT#: TA349021

Rabbit Polyclonal Anti-Alpha-tubulin Antibody

Product Images

Western blot analysis of alpha-tubulin in human brain tissue lysate with alpha-tubulin antibody at (A) 0.25 and (B) 0.5 ug/mL.

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  Immunohistochemistry of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 5 ug/mL.

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  Immunofluorescence of Alpha-tubulin in human brain tissue with Alpha-tubulin antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.25 - 0.5 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Alpha-tubulin antibody was raised against an 18 amino acid peptide near the amino terminus of human alpha-tubulin
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Alpha-tubulin antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 50 kDa; Observed: 45 kDa
• Gene Name: transient receptor potential cation channel subfamily C member 3
• Database Link:
  NP_003296
  Entrez Gene 22065 MouseEntrez Gene 60395 RatEntrez Gene 7222 Human
  UniProt ID Q13507
• Background: Alpha-tubulin belongs to the tubulin superfamily, which is composed of six distinct families. Along with beta-tubulins, alpha-tubulins are the major components of microtubules. These microtubules are involved in a wide variety of cellular activities ranging from mitosis and transport events to cell movement and the maintenance of cell shape. Alpha- and beta-tubulin dimers are assembled to 13 protofilaments that form a microtubule of 22-nm diameter (reviewed in 1). Tyrosine ligase adds a C-terminal tyrosine to monomeric alpha-tubulin. Assembled microtubules can again be detyrosinated by a cytoskeleton-associated carboxypeptidase (2). Another post-translational modification of detyrosinated alpha-tubulin is C-terminal polyglutamylation, which is characteristic of microtubules in neuronal cells and the mitotic spindle (3). Like GAPDH and ?-Actin, this antibody makes an excellent loading control in immunoblots.
• Synonyms: SCA41; TRP3

Reference Data

• Protein Families: Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane