USP25 Rabbit Polyclonal Antibody

CAT#: TA349043

Rabbit Polyclonal Anti-USP25 Antibody

Product Images

Western blot analysis of USP25 in mouse brain tissue lysate with USP25 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of USP25 in human brain tissue with USP25 antibody at 5 ug/mL.

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  Immunofluorescence of USP25 in human brain tissue with USP25 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: USP25 antibody was raised against a 17 amino acid peptide near the center of human USP25.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: USP25 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 116 kDa; Observed: 105 kDa
• Gene Name: ubiquitin specific peptidase 25
• Database Link:
  NP_001269970
  Entrez Gene 30940 MouseEntrez Gene 29761 Human
  UniProt ID Q9UHP3
• Background: USP25 (ubiquitin specific peptidase 25), also known as USP21, belongs to the peptidase C19 family and is a highly conserved 76-amino acid protein involved in regulation of intracellular protein breakdown, cell cycle regulation, and stress response (1). It contains one UBA-like domain and two UIM (ubiquitin-interacting motif) repeats. Due to alternative splicing events, USP25 is expressed as two short, ubiquitously expressed isoforms and one long, muscle-specific isoform (2). The long isoform of USP25 (USP25m) is upregulated in myogenesis and is implicated in regulation of muscular differentiation and function. USP25 is a deubiquitinating enzyme (DUB) that negatively regulates IL-17-triggered signaling (3,4).
• Synonyms: USP21

Reference Data

• Protein Families: Protease