SMURF1 Rabbit Polyclonal Antibody

CAT#: TA349048

Rabbit Polyclonal Anti-MIB1 Antibody

Product Images

Western blot analysis of MIB1 in A431 cell lysate with MIB1 antibody at 1 ug/mL.

• 

  Immunocytochemistry of MIB1 in A431 cells with MIB1 antibody at 5 ug/mL.

• 

  Immunofluorescence of MIB1 in A431 cells with MIB1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: MIB1 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human MIB1.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: MIB1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 89, 102, 111 kDa; Observed: 103 kDa
• Gene Name: SMAD specific E3 ubiquitin protein ligase 1
• Database Link:
  NP_851994
  Entrez Gene 75788 MouseEntrez Gene 690516 RatEntrez Gene 57154 Human
  UniProt ID Q9HCE7
• Background: The E3 ubiquitin-protein ligase MIB1, also known as Mindbomb homolog 1, contains multiple ankyrin repeats and RING finger domains that functions as an E3 ubiquitin ligase (1). MIB1 interacts with and regulates the cellular expression of the death-associated protein kinase 1 (DAPK1) protein by promoting its ubiquitination and degradation (1). It also positively regulates Notch signaling by ubiquitinating the Notch receptors, thereby facilitating their endocytosis (2).
• Synonyms: KIAA1625

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Endocytosis, TGF-beta signaling pathway, Ubiquitin mediated proteolysis