SMURF 2 (SMURF2) Rabbit Polyclonal Antibody

CAT#: TA349051

Rabbit Polyclonal Anti-DEPTOR Antibody

Product Images

Western blot analysis of DEPTOR in mouse liver tissue lysate with DEPTOR antibody at (A) 1 and (B) 2 ug/mL.

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  Immunohistochemistry of DEPTOR in mouse liver tissue with DEPTOR antibody at 5 ug/mL.

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: DEPTOR antibody was raised against a 16 amino acid peptide near the center of human DEPTOR.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: DEPTOR antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 45 kDa; Observed: 42 kDa
• Gene Name: SMAD specific E3 ubiquitin protein ligase 2
• Database Link:
  NP_073576
  Entrez Gene 66313 MouseEntrez Gene 303614 RatEntrez Gene 64750 Human
  UniProt ID Q9HAU4
• Background: DEPTOR is an mTOR (mammalian target of rapamycin)-interacting protein whose expression is negatively regualted by mTOR complex 1 (mTORC1) and mTORC2 (1). Loss of DEPTOR activates RP6SK1, Akt1, and SGK1, promotes cell growth and survival, and activates mTORC1 and mTORC2 kinase activities. DEPTOR expression is low in most cancers, but is often elevated in multiple myelomas (1). In respons to stimulation by mitogens, DEPTOR is rapidly phosphorylated leading to its ubiquitination and degradation, allowing mTOR to generate an auto-amplification loop to promotes its own full activation (2).
• Synonyms: DKFZp686F0270; MGC138150

Reference Data

• Protein Families: Adult stem cells, Druggable Genome, ES Cell Differentiation/IPS, Transcription Factors
• Protein Pathways: Allograft rejection, Antigen processing and presentation, Autoimmune thyroid disease, Cell adhesion molecules (CAMs), Endocytosis, Graft-versus-host disease, TGF-beta signaling pathway, Type I diabetes mellitus, Ubiquitin mediated proteolysis, Viral myocarditis