PI 3 Kinase regulatory subunit 4 (PIK3R4) Rabbit Polyclonal Antibody

CAT#: TA349083

Rabbit Polyclonal Anti-NOX4 Antibody

Product Images

Western blot analysis of NOX4 in Jurkat cell lysate with NOX4 antibody at 1 ug/mL in (A) the absence and (B) the presence of blocking peptide.

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  Immunohistochemistry of NOX4 in human spleen tissue with NOX4 antibody at 5 ug/mL.

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  Immunofluorescence of NOX4 in human spleen tissue with NOX4 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: NOX4 antibody was raised against a 14 amino acid peptide near the amino terminus of human NOX4.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: NOX4 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 64 kDa; Observed: 68 kDa
• Gene Name: phosphoinositide-3-kinase regulatory subunit 4
• Database Link:
  NP_055417
  Entrez Gene 75669 MouseEntrez Gene 363131 RatEntrez Gene 30849 Human
  UniProt ID Q99570
• Background: The NOX family of NAPDH oxidases is comprised of seven transmembrane proteins that oxidize intracellular NAPDH/NADH, causing electron transport across the membrane and the reduction of molecular oxygen to superoxide (1). NOX4 is is expressed in multiple tissues and catalyzes the reduction of molecular oxygen to various reactive oxygen species (ROS) (2,3). Unlike other NOX proteins, NOX4 does not require cytosolic subunits and thus is constitutively active (4). The function of NOX4 remains unclear as it plays both protective and deleterious roles in cellular metabolism.
• Synonyms: p150; VPS15

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Regulation of autophagy