ERp72 (PDIA4) Rabbit Polyclonal Antibody

CAT#: TA349091

Rabbit Polyclonal Anti-Rubicon Antibody

Product Images

Western blot analysis of Rubicon in 293 cell lysate with Rubicon antibody at 1 ug/mL.

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  Immunohistochemistry of RUBICON in human kidney tissue with RUBICON antibody at 5 ug/mL.

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  Immunofluorescence of RUBICON in human kidney tissue with RUBICON antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 1 - 2 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Rubicon antibody was raised against a 16 amino acid peptide near the amino terminus of human Rubicon.
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: Rubicon antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 90, 101, 109 kDa; Observed: 91, 99 kDa
• Gene Name: protein disulfide isomerase family A member 4
• Database Link:
  NP_004902
  Entrez Gene 12304 MouseEntrez Gene 116598 RatEntrez Gene 9601 Human
  UniProt ID P13667
• Background: Two Beclin-1-interacting proteins, the run domain Beclin-1 interacting and cysteine-rich containing protein (Rubicon) and ATG14L, reciprocally regulate autophagy at different stages. Knockdown of Rubicon caused enhancement of autophagy while that of ATG14L caused a defect in autophagosome formation (1). Rubicon functions as part of a Beclin-1-PIK3C3-containing autophagy complex and is also an essential, positive regulator of the NAPDH oxidase complex (2). Upon microbial infection or TLR2 activation, Rubicon interacts with the CYBA subunit of the NAPDH oxidase complex, leading to a burst of reactive oxygen species and inflammatory cytokines (2).
• Synonyms: ERp-72; ERP70; ERP72

Reference Data

• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Vibrio cholerae infection