CRBN Rabbit Polyclonal Antibody

CAT#: TA349125

Rabbit Polyclonal Anti-LIMA1 Antibody

Product Images

Western blot analysis of LIMA1 in human colon tissue lysate with LIMA1 antibody at (A) 0.5 and (B) 1 ug/mL.

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  Immunohistochemistry of LIMA1 in human colon tissue with LIMA1 antibody at 5 ug/mL.

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  Immunofluorescence of LIMA1 in human colon tissue with LIMA1 antibody at 20 ug/mL.

Specifications

Product Data

• Applications: IF, IHC, WB
• Recommended Dilution: WB: 0.5 - 1 ug/mL, IHC: 5 ug/mL, IF: 20 ug/mL
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: LIMA1 antibody was raised against an 18 amino acid peptide near the amino terminus of human LIMA1
• Formulation: PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Purification: LIMA1 antibody is affinity chromatography purified via peptide column.
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: Predicted: 50, 66, 84 kDa; Observed: 65 kDa
• Gene Name: cereblon
• Database Link:
  NP_057386
  Entrez Gene 51185 Human
  UniProt ID Q96SW2
• Background: The Lim domain and actin-binding protein 1 (LIMA1) is cytoskeleton-associated protein that inhibits actin filament depolymerization and cross-links filaments in bundles and is downregulated in some cancer cell lines (1,2). LIMA1 is also a key molecule linking the cadherin-catenin complex to the actin cytoskeleton (3). Recent studies have shown that EGF activates ERK1/2-dependent phosphorylation, ubiquitination and degradation of LIMA1, leading to increased invasiveness and metastasis of metastatic prostate cancer models (4).
• Synonyms: MRT2; MRT2A

Reference Data

• Protein Families: Protease