beta Actin (ACTB) Rabbit Polyclonal Antibody

CAT#: TA349205

Rabbit Polyclonal Anti-ACTB Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: Mouse kidney tissue Mouse brain tissue 231 Hela 293T and HepG2 cell lysates Primary antibody: TA349205 (ACTB Antibody) at dilution 1/1600 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 2 minutes

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349205 (ACTB Antibody) at dilution 1/50 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA349205 (ACTB Antibody) at dilution 1/50, treated with synthetic peptide. (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA349205 (ACTB Antibody) at dilution 1/50 (Original magnification: ×200)

• 

  Immunohistochemistry of paraffin-embedded Human tonsil tissue using TA349205 (ACTB Antibody) at dilution 1/50, treated with synthetic peptide. (Original magnification: ×200)

Show all 5 images

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: Mouse kidney tissue, Mouse brain tissue, 231, Hela, 293T and HepG2 cell lysates
  IHC: 50-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human ACTB
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 42 kDa
• Gene Name: actin, beta
• Database Link:
  NP_001092
  Entrez Gene 11461 MouseEntrez Gene 81822 RatEntrez Gene 60 Human
  UniProt ID P60709
• Background: This gene encodes one of six different actin proteins. Actins are highly conserved proteins that are involved in cell motility, structure, and integrity. This actin is a major constituent of the contractile apparatus and one of the two nonmuscle cytoskeletal actins. Polymerization of globular actin (G-actin) leads to a structural filament (F-actin) in the form of a two-stranded helix. Each actin can bind to 4 others. Identified in a mRNP granule complex, at least composed of ACTB, ACTN4, DHX9, ERG, HNRNPA1, HNRNPA2B1, HNRNPAB, HNRNPD, HNRNPL, HNRNPR, HNRNPU, HSPA1, HSPA8, IGF2BP1, ILF2, ILF3, NCBP1, NCL, PABPC1, PABPC4, PABPN1, RPLP0, RPS3, RPS3A, RPS4X, RPS8, RPS9, SYNCRIP, TROVE2, YBX1 and untranslated mRNAs.
• Synonyms: BRWS1; PS1TP5BP1

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adherens junction, Arrhythmogenic right ventricular cardiomyopathy (ARVC), Dilated cardiomyopathy, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Leukocyte transendothelial migration, Pathogenic Escherichia coli infection, Regulation of actin cytoskeleton, Tight junction, Vibrio cholerae infection, Viral myocarditis