GAPDH Rabbit Polyclonal Antibody

CAT#: TA349207

Rabbit Polyclonal Anti-GAPDH Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-5: Hela 293T Jurkat A431 A549 cell lysates Primary antibody: TA349207 (GAPDH Antibody) at dilution 1/4000 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA349207 (GAPDH Antibody) at dilution 1/70 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA349207 (GAPDH Antibody) at dilution 1/70, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA349207 (GAPDH Antibody) at dilution 1/70 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA349207 (GAPDH Antibody) at dilution 1/70, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 2000-10000
  WB positive control: Hela, 293T, Jurkat, A431, A549 cell lysates
  IHC: 50-200
  Positive control: Human esophagus cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human GAPDH
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 37 kDa
• Gene Name: glyceraldehyde-3-phosphate dehydrogenase
• Database Link:
  NP_002037
  Entrez Gene 14433 MouseEntrez Gene 24383 RatEntrez Gene 2597 Human
  UniProt ID P04406
• Background: The abundance of glyceraldehyde-3-phosphate dehydrogenase in eukaryotic cells is relatively unaffected by eternal factors. In western blot analysis the level of GAPDH, a 38kDa protein, can be used as a reference value for loading control. Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC By similarity. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate.
• Synonyms: G3PD; GAPD; HEL-S-162eP

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Alzheimer's disease, Glycolysis / Gluconeogenesis, Metabolic pathways