MCM2 Rabbit Polyclonal Antibody

CAT#: TA349394

Rabbit Polyclonal Anti-MCM2 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-5: 293T cells hela cells K562 cells Raji cells 231 cells Primary antibody: TA349394 (MCM2 Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 second

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349394 (MCM2 Antibody) at dilution 1/20 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349394 (MCM2 Antibody) at dilution 1/20, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 293T, hela, K562, Raji and 231 cells
  IHC: 25-100
  Positive control: Human lung cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human MCM2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 102 kDa
• Gene Name: minichromosome maintenance complex component 2
• Database Link:
  NP_004517
  Entrez Gene 17216 MouseEntrez Gene 4171 Human
  UniProt ID P49736
• Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein forms a complex with MCM4, 6, and 7, and has been shown to regulate the helicase activity of the complex. This protein is phosphorylated, and thus regulated by, protein kinases CDC2 and CDC7. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined.
• Synonyms: BM28; CCNL1; cdc19; CDCL1; D3S3194; MITOTIN

Reference Data

• Protein Families: Druggable Genome, Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Cell cycle, DNA replication