MGMT Rabbit Polyclonal Antibody

CAT#: TA349396

Rabbit Polyclonal Anti-MGMT Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane: Raji cells Primary antibody: TA349396 (MGMT Antibody) at dilution 1/150 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Raji cells
• Reactivities: Human
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human MGMT
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 22 kDa
• Gene Name: O-6-methylguanine-DNA methyltransferase
• Database Link:
  NP_002403
  Entrez Gene 4255 Human
  UniProt ID P16455
• Background: MGMT (O6-methylguanine-DNA methyltransferase) is transcriptionally activated in response to DNA damage and functions to repair mutagenic and cytotoxic O6-alkylguanine lesions caused by carcinogens or cytostatic drugs. MGMT induction by ionising radiation does not occur in p53-deficient mice, suggesting that MGMT induction may require p53. Similarly, MGMT mRNA and protein were shown to be inducible by ionising radiation, only in cell lines that express functional p53, and not in cell lines that do not express wild type p53. In contrast, high MGMT activity was associated with the presence of mutant p53, in a study of oral cancer cell lines. Similarly, MGMT activity was significantly lower in ovarian tumors with wildtype p53 than in tumors with mutant p53, supporting the view that wildtype p53 down-regulates the basal MGMT promoter.
• Synonyms: methylguanine-DNA methyltransferase; O-6-methylguanine-DNA methyltransferase; O6-methylguanine-DNA methyltransferase; OTTHUMP00000020741

Reference Data

• Protein Families: Druggable Genome