ARFGAP1 Rabbit Polyclonal Antibody

CAT#: TA349499

Rabbit Polyclonal Anti-ARFGAP1 Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-4: 293T cells A549 cells PC3 cells NIH/3T3 cells Primary antibody: TA349499 (ARFGAP1 Antibody) at dilution 1/600 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 seconds

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349499 (ARFGAP1 Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA349499 (ARFGAP1 Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: 293T, A549, PC3 and NIH/3T3 cells
  IHC: 25-100
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ARFGAP1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 45 kDa
• Gene Name: ADP ribosylation factor GTPase activating protein 1
• Database Link:
  NP_783202
  Entrez Gene 228998 MouseEntrez Gene 55738 Human
  UniProt ID Q8N6T3
• Background: The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene.
• Synonyms: ARF1GAP; HRIHFB2281

Reference Data

• Protein Pathways: Endocytosis