ASAH2 Rabbit Polyclonal Antibody

CAT#: TA349659

Rabbit Polyclonal Anti-ASAH2 Antibody

Product Images

Gel: 10%SDS-PAGE Lysate: 40 μg Lane 1-2: Lovo cells mouse heart tissue Primary antibody: TA349659 (ASAH2 Antibody) at dilution 1/270 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA349659 (ASAH2 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human brain tissue using TA349659 (ASAH2 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA349659 (ASAH2 Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA349659 (ASAH2 Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Lovo cells and mouse heart tissue
  IHC: 25-100
  Positive control: Human brain
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human ASAH2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 86 kDa
• Gene Name: N-acylsphingosine amidohydrolase (non-lysosomal ceramidase) 2
• Database Link:
  NP_063946
  Entrez Gene 54447 MouseEntrez Gene 114104 RatEntrez Gene 56624 Human
  UniProt ID Q9NR71
• Background: Ceramidases (EC 3.5.1.23), such as ASAH2, catalyze hydrolysis of the N-acyl linkage of ceramide, a second messenger in a variety of cellular events, to produce sphingosine. Sphingosine exerts both mitogenic and apoptosis-inducing activities, and its phosphorylated form functions as an intra- and intercellular second messenger.
• Synonyms: BCDase; HNAC1; LCDase; N-CDase; NCDase

Reference Data

• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism