HMGCL Rabbit Polyclonal Antibody

CAT#: TA350049

Rabbit Polyclonal Anti-HMGCL Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane 1-6: Mouse thymus tissue human ovarian cancer tissue mouse heart tissue human fetal liver tissue mouse liver tissue SKOV3 cells Primary antibody: TA350049 (HMGCL Antibody) at dilution 1/300 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 5 seconds

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA350049 (HMGCL Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA350049 (HMGCL Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350049 (HMGCL Antibody) at dilution 1/30 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350049 (HMGCL Antibody) at dilution 1/30, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse thymus and human ovarian cancer tissue, mouse heart, human fetal liver, mouse liver tissue and SKOV3 cells
  IHC: 50-200
  Positive control: Human ovarian cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human HMGCL
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: 3-hydroxymethyl-3-methylglutaryl-CoA lyase
• Database Link:
  NP_000182
  Entrez Gene 15356 MouseEntrez Gene 79238 RatEntrez Gene 3155 Human
  UniProt ID P35914
• Background: The protein encoded by this gene belongs to the HMG-CoA lyase family. It is a mitochondrial enzyme that catalyzes the final step of leucine degradation and plays a key role in ketone body formation. Mutations in this gene are associated with HMG-CoA lyase deficiency. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
• Synonyms: HL

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Butanoate metabolism, Metabolic pathways, Synthesis and degradation of ketone bodies, Valine, leucine and isoleucine degradation