IBRDC2 (RNF144B) Rabbit Polyclonal Antibody

CAT#: TA350073

Rabbit Polyclonal Anti-RNF144B Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: TM4 cells Primary antibody: TA350073 (RNF144B Antibody) at dilution 1/400 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 3 minutes

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA350073 (RNF144B Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TA350073 (RNF144B Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350073 (RNF144B Antibody) at dilution 1/50 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using TA350073 (RNF144B Antibody) at dilution 1/50, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: TM4 cells
  IHC: 50-200
  Positive control: Human lung cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human RNF144B
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 34 kDa
• Gene Name: ring finger protein 144B
• Database Link:
  NP_877434
  Entrez Gene 218215 MouseEntrez Gene 255488 Human
  UniProt ID Q7Z419
• Background: p53 is the most commonly mutated gene in human cancer identified to date. Expression of p53 leads to inhibition of cell growth by preventing progression of cells from G1 to S phase of the cell cycle. Most importantly, p53 functions to cause arrest of cells in the G1 phase of the cell cycle following any exposure of cells to DNA-damaging agents. The MDM2 (murine double minute-2) protein was initially identified as an oncogene in a murine transformation system. MDM2 functions to bind p53 and block p53-mediated transactivation of cotransfected reporter constructs. The MDM2 gene is amplified in a high percentage of human sarcomas that retain wildtype p53 and tumor cells that overexpress MDM2 can tolerate high levels of p53 expression. Another p53 target protein is the p53-inducible RING finger protein (p53RFP), an auto-ubiquitinylated protein acting as an E3 ubiquitin ligase. p53RFP, also designated IBRDC2 in mouse and rat, receives ubiquitin from specific E2 ubiquitin-conjugating enzymes and transfers it to substrates that promote their degradation by the proteasome. p53RFP may mediate re-entry into the cell cycle.
• Synonyms: bA528A10.3; IBRDC2; p53RFP; PIR2

Reference Data

• Protein Families: Transmembrane