PCK2 Rabbit Polyclonal Antibody

CAT#: TA350174

Rabbit Polyclonal Anti-PCK2 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-3: 293T cells Jurkat cells Hela cells Primary antibody: TA350174 (PCK2 Antibody) at dilution 1/750 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA350174 (PCK2 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TA350174 (PCK2 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350174 (PCK2 Antibody) at dilution 1/60 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human colon cancer tissue using TA350174 (PCK2 Antibody) at dilution 1/60, treated with fusion protein. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: 293T, Jurkat and Hela cells
  IHC: 100-300
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human PCK2
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 71 kDa
• Gene Name: phosphoenolpyruvate carboxykinase 2, mitochondrial
• Database Link:
  NP_004554
  Entrez Gene 74551 MouseEntrez Gene 5106 Human
  UniProt ID Q16822
• Background: This gene encodes a mitochondrial enzyme that catalyzes the conversion of oxaloacetate to phosphoenolpyruvate in the presence of guanosine triphosphate (GTP). A cytosolic form of this protein is encoded by a different gene and is the key enzyme of gluconeogenesis in the liver. Alternatively spliced transcript variants have been described.
• Synonyms: PEPCK; PEPCK-M; PEPCK2

Reference Data

• Protein Families: ES Cell Differentiation/IPS
• Protein Pathways: Adipocytokine signaling pathway, Citrate cycle (TCA cycle), Glycolysis / Gluconeogenesis, Insulin signaling pathway, Metabolic pathways, PPAR signaling pathway, Pyruvate metabolism