Myosin Phosphatase (PPP1R12A) Rabbit Polyclonal Antibody

CAT#: TA350197

Rabbit Polyclonal Anti-PPP1R12A Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane 1-4: NIH/3T3 cells A172 cells hela cells PC3 cells Primary antibody: TA350197 (PPP1R12A Antibody) at dilution 1/350 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 40 seconds

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350197 (PPP1R12A Antibody) at dilution 1/25 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using TA350197 (PPP1R12A Antibody) at dilution 1/25, treated with fusion protein. (Original magnification: ×200)

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 1000-5000
  WB positive control: NIH/3T3, A172, hela and PC3 cells
  IHC: 25-100
  Positive control: Human thyroid cancer
  Predicted cell location: Cytoplasm
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Fusion protein of human PPP1R12A
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 115 kDa
• Gene Name: protein phosphatase 1 regulatory subunit 12A
• Database Link:
  NP_002471
  Entrez Gene 17931 MouseEntrez Gene 116670 RatEntrez Gene 4659 Human
  UniProt ID O14974
• Background: Myosin phosphatase target subunit 1, which is also called the myosin-binding subunit of myosin phosphatase, is one of the subunits of myosin phosphatase. Myosin phosphatase regulates the interaction of actin and myosin downstream of the guanosine triphosphatase Rho. The small guanosine triphosphatase Rho is implicated in myosin light chain (MLC) phosphorylation, which results in contraction of smooth muscle and interaction of actin and myosin in nonmuscle cells. The guanosine triphosphate (GTP)-bound, active form of RhoA (GTP.RhoA) specifically interacted with the myosin-binding subunit (MBS) of myosin phosphatase, which regulates the extent of phosphorylation of MLC.
• Synonyms: M130; MBS; MYPT1

Reference Data

• Protein Families: Druggable Genome
• Protein Pathways: Focal adhesion, Long-term potentiation, Regulation of actin cytoskeleton, Vascular smooth muscle contraction