Chk1 (CHEK1) Rabbit Polyclonal Antibody

CAT#: TA350636

Rabbit Polyclonal Anti-CHEK1 Antibody

Product Images

Gel: 8%SDS-PAGE Lysate: 40 μg Lane: A431 cells Primary antibody: TA350636 (CHEK1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 10 minutes

Specifications

Product Data

• Applications: WB
• Recommended Dilution: WB: 200-1000
  WB positive control: A431 cells
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human CHEK1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 54 kDa
• Gene Name: checkpoint kinase 1
• Database Link:
  NP_001265
  Entrez Gene 12649 MouseEntrez Gene 140583 RatEntrez Gene 1111 Human
  UniProt ID O14757
• Background: The protein encoded by this gene belongs to the Ser/Thr protein kinase family. It is required for checkpoint mediated cell cycle arrest in response to DNA damage or the presence of unreplicated DNA. This protein acts to integrate signals from ATM and ATR, two cell cycle proteins involved in DNA damage responses, that also associate with chromatin in meiotic prophase I. Phosphorylation of CDC25A protein phosphatase by this protein is required for cells to delay cell cycle progression in response to double-strand DNA breaks. Several alternatively spliced transcript variants have been found for this gene.
• Synonyms: CHK1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase, Stem cell - Pluripotency
• Protein Pathways: Cell cycle, p53 signaling pathway