AMPK alpha 1 (PRKAA1) Rabbit Polyclonal Antibody

CAT#: TA350851

Rabbit Polyclonal Anti-PRKAA1 Antibody

Product Images

Gel: 6%SDS-PAGE Lysate: 40 μg Lane: NIH/3T3 cells Primary antibody: TA350851 (PRKAA1 Antibody) at dilution 1/200 Secondary antibody: Goat anti rabbit IgG at 1/8000 dilution Exposure time: 1 minute

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA350851 (PRKAA1 Antibody) at dilution 1/80 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using TA350851 (PRKAA1 Antibody) at dilution 1/80, treated with synthetic peptide. (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA350851 (PRKAA1 Antibody) at dilution 1/80 (Original magnification: ×200)

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  Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using TA350851 (PRKAA1 Antibody) at dilution 1/80, treated with synthetic peptide. (Original magnification: ×200)

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Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 200-1000
  WB positive control: Human right lung and left lung cancer tissue
  IHC: 100-300
  Positive control: Human liver cancer
  Predicted cell location: Nucleus
• Reactivities: Human, Mouse, Rat
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human PRKAA1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 64 kDa
• Gene Name: protein kinase AMP-activated catalytic subunit alpha 1
• Database Link:
  NP_996790
  Entrez Gene 65248 RatEntrez Gene 105787 MouseEntrez Gene 5562 Human
  UniProt ID Q13131
• Background: The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
• Synonyms: AMPK; AMPKa1

Reference Data

• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway, Hypertrophic cardiomyopathy (HCM), Insulin signaling pathway, mTOR signaling pathway, Regulation of autophagy