HIF1AN Rabbit Polyclonal Antibody

CAT#: TA351582

Rabbit Polyclonal Anti-HIF1AN Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Mouse brain and liver tissue
  IHC: 50-300
  Positive control: Human esophagus cancer
  Predicted cell location: Cytoplasm and Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human HIF1AN
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 40 kDa
• Gene Name: hypoxia inducible factor 1 alpha subunit inhibitor
• Database Link:
  NP_060372
  Entrez Gene 319594 MouseEntrez Gene 55662 Human
  UniProt ID Q9NWT6
• Background: Factor inhibiting HIF-1 (FIH-1) exists as a homodimer and binds to HIF-1alpha. Specifically, FIH-1 operates as an asparaginyl hydroxylase. It catalyzes the hydroxylation of the beta-carbon of Asparagine residue 803 within the carboxy terminal transactivation domain of HIF-1alpha. This hydroxylation event blocks the association of HIF-1alpha with co-activators. FIH-1 also binds to von Hippel-Lindau (VHL) tumor suppressor protein, which represses transcriptional ac-tivity of HIF-1alpha. In transiently transfected human osteosarcoma cells, FIH-1 localizes to the cytoplasm.
• Synonyms: FIH1

Reference Data

• Protein Families: Druggable Genome