SRGAP1 Rabbit Polyclonal Antibody

CAT#: TA351752

Rabbit Polyclonal Anti-SRGAP1 Antibody

Specifications

Product Data

• Applications: IHC, WB
• Recommended Dilution: WB: 500-2000
  WB positive control: Hepg2, TM4, 293T and NIH/3T3 cells
  IHC: 25-100
  Positive control: Human liver cancer
  Predicted cell location: Cytoplasm or Nucleus
• Reactivities: Human, Mouse
• Host: Rabbit
• Isotype: IgG
• Clonality: Polyclonal
• Immunogen: Synthetic peptide of human SRGAP1
• Formulation: pH7.4 PBS, 0.05% NaN3, 40% Glyceroln
• Concentration: lot specific
• Purification: Antigen affinity purification
• Conjugation: Unconjugated
• Storage: Store at -20°C as received.
• Stability: Stable for 12 months from date of receipt.
• Predicted Protein Size: 124 kDa
• Gene Name: SLIT-ROBO Rho GTPase activating protein 1
• Database Link:
  NP_065813
  Entrez Gene 117600 MouseEntrez Gene 57522 Human
  UniProt ID Q7Z6B7
• Background: SRGAP1 contains an FCH (Fes/CIP4 homology) domain, a Rho-GAP domain and an SH3 domain. In the presence of Slit, SRGAP1 (via its SH3 domain) binds to the CC3 motif in robo (a protein responsible for mediating the repulsive effect of Slit) with higher affinity and inhibits Cdc42 activity in a robo/SRGAP-dependent manner. More specifically, SRGAP1 increases the intrinsic GTPase activity of Cdc42, thereby converting it to its inactive, GDP-bound form. Inactivation of Cdc42 ultimately leads to a decrease in actin polymerization.
• Synonyms: ARHGAP13; NMTC2

Reference Data

• Protein Pathways: Axon guidance